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Addition of the neurotrophic factor to in vitro cultures of rat cortical neurons has been shown to cause enhancement of the spontaneous electrical activity and maturation of synaptic contacts at the neuromuscular junctions, the postsynaptic densities and AChE-positive membranes. The effect of the neurotrophic factor on neuronal maturation is apparently mediated by axonal transport mechanisms. The role of axonal transport of neurotrophic factor in the effect of neurotrophic-active substances on the maturation of neurons was studied in the present work. Addition of the neurotrophic factor to the culture medium enhanced the maturation of neurons. A specific peptidase inhibitor, bestatin (50 microM), a colchicine-binding inhibitor, colchicine (10 microM) and the toxin MPP3 (1 microM) caused modification of the effect of the neurotrophic factor on neuronal maturation. The peptidase inhibitor revealed suppressive and colchicine revealed stimulatory effects on neurite outgrowth and formation of neuronal networks. The data obtained suggest that a greater involvement of the fast axonal transport of neurotrophic factor in maturation of neurons can be attributed to a continuous formation of the slow type of axonal transport in the culture medium.HPG İngilizcesi Çalışmaları Üsteğenmiş Bütün Finans Girişimleri Yaparken
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