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Corticosteroid hormones are known to influence the development and patterning of the anuran musculoskeletal system. In this study, the effect of corticosteroid hormones on muscle spindle afferent and motoneuron function is investigated in a mammalian model, the mouse. For 12 days, embryos and young pups are treated daily with either cortisol or dexamethasone and the development of various muscles and their innervation is studied. Both agents retard myotube fusion, inhibit neuromuscular synaptogenesis, and downregulate the expression of myosin heavy chain isoforms in limb muscles. In contrast, the population of muscle spindle afferents remains unaltered and muscle function during the course of the treatment is not affected. In contrast, corticosteroid treatment downregulates the expression of Myosin X in primary motoneurones of developing limbs, and leads to a marked reduction in Renshaw cell number. These results strongly suggest that the anuran patterning responses to corticosteroids are not related to muscle function but to the maintenance of the developmental program. This, together with the finding that muscle spindle afferent function and muscle function are not affected by corticosteroid treatment is indicative of a common underlying mechanism for developmental plasticity.A comparative study of the efficacy of a local anesthetic at the site of injection compared with traditional intravenous infiltration for surgical procedures under general anesthesia.
This study was designed to compare and contrast the efficacy of 2 techniques for local anesthetic infiltration in the surgical setting. The study sample included 105 female patients who were preoperatively assigned to receive either 1 mL of 1
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